Journal: Scientific Reports
Article Title: Epimedin C attenuates airway inflammation and remodeling in Asthma by intervening M2 macrophage polarization via modulating the PI3K/Akt/mTOR signaling pathway
doi: 10.1038/s41598-026-42160-4
Figure Lengend Snippet: Epimedin C inhibited M2 macrophage markers, pro-inflammatory, and pro-EMT factors in vitro. ( A )Immunofluorescence detected Arg-1 in BMDMs; ( B ) Immunofluorescence detected CD206 in BMDMs; ( C ) RT-qPCR detected mRNA relative expression of Arg-1 and CD206; ( D ) RT-qPCR detected mRNA relative expression of VEGF and TGF-β1; (E) ELISA detected VEGF and TGF-β1 in the BMDMs supernatant. n = 8 for A ~ E. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 compared to control group. ### P < 0.001 and #### P < 0.0001 compared to the IL-4 group. & P < 0.05, && P < 0.01, and &&& P < 0.001 compared between annotated groups. Arg-1, Arginase-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; BMDM, bone marrow-derived macrophage.
Article Snippet: Mouse bone marrow-derived macrophages (BMDM) purchased from Procell (CP-M141; Wuhan, China) were cultured in BMDM complete medium (CM-M141; Procell) containing 10% foetal bovine serum (FBS; Hyclone).
Techniques: In Vitro, Immunofluorescence, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Control, Derivative Assay